Vivek Jedhe, Suraj Adole, Sanjiv Patil and Bhuvaneshwari Mehere
Published in Vol.15, 2025
KEYWORDS: Selenium nanoparticles, Moringa oleifera, Berberis aristata, antimicrobial activity, Gram-positive, Gram-negative, green synthesis.
ABSTRACT:
Green synthesis of metal containing nanoparticles using medicinal plant parts extract has emerged as an effective strategy to combat multidrug-resistant pathogens. This study investigates the antimicrobial potential of selenium nanoparticles (SeNPs) synthesized from Moringa oleifera leaf extract (MOSeNPs) and Berberis aristata root extract (BASeNPs) against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). SeNPs were synthesized using aqueous plant extracts as both reducing and stabilizing agents and characterized using sophisticated instrumentation techniques like UV-Vis spectroscopy, SEM, TEM, FTIR, and XRD. Antimicrobial activity was assessed via agar well diffusion and Minimum Inhibitory Concentration (MIC) was determined using the broth microdilution method. Both MOSeNPs and BASeNPs exhibited dose-dependent inhibition zones. BASeNPs demonstrated slightly higher efficacy against S. aureus, while MOSeNPs showed strong, broad-spectrum activity. MIC values ranged from 8–64 μg/mL, confirming substantial bacteriostatic activity. These findings suggest SeNPs synthesized via green methods possess significant antibacterial properties and offer potential as alternative therapeutics against drug-resistant bacteria.
DOI: https://doi.org/10.64833/hislopia.j.vol.15.issue1.50-60